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高效液相色谱法测定乳汁和血浆中的氟比洛芬浓度
Determination of flurbiprofen in maternal breast milk and plasma by HPLC
目的：建立高效液相色谱法检测产妇母乳和血浆中氟比洛芬的方法，用于评估产妇乳汁/血药浓度百分比。方法：以卡马西平为内标，乳汁和血浆样品加入1 mol·L-1的盐酸溶液后用正己烷：乙酸乙酯（1：4）萃取，选择C18柱检测，以甲醇-6.85 mmol·L-1磷酸-乙腈（30：40：30）为流动相，流速为1.0 mL·min-1，检测波长为247 nm，柱温为30℃。结果：乳汁和血浆中氟比洛芬的浓度分别在0.02~5.00 μg·mL-1（R2=0.999 7）和0.05~10.00 μg·mL-1（R2=0.999 2）内线性关系良好，日内、日间RSD均低于6%。20例产妇24，36，48 h的乳汁/血药浓度百分比分别为6.87%，6.33%，6.58%。结论：该方法快速、简便、灵敏度高、重现性好。氟比洛芬在乳汁中分布较少。
OBJECTIVE To establish a HPLC method for the determination of flurbiprofen in maternal breast milk and plasma, and assess the milk-to-plasma ratio in pregnant women.METHODS With carbamazepine as internal standard, milk and plasma samples were added to 1 mol·L-1 hydrochloric acid solution and were extracted with hexane:ethyl acetate (1:4). The analysis was conducted on C18 column, with the mobile phase of methanol-6.85 mmol·L-1 phosphoric acid-acetonitrile (30:40:30), the flow rate at 1.0 mL·min-1, the detection wavelength at 247 nm, and the column temperature at 30℃.RESULTS Milk and plasma concentrations of flurbiprofen were 0.02-5.00 μg·mL-1 (R2=0.999 7) and 0.05-10.00 μg·mL-1 (R2=0.999 2), in good linear relationship. The intra-and inter-day precisions were both less than 6%.The milk-to-plasma ratios were 6.87%, 6.63% and 6.58% in 20 pregnant women at 24, 36, 48 h.CONCLUSION The method is rapid, simple, has high sensitivity and good reproducibility. The breast milk only contains a little flurbiprofen.
氟比洛芬 / 高效液相色谱法 / 血药浓度 / 乳药浓度 / 产妇 {{custom_keyword}} /
flurbiprofen / HPLC / blood concentration / concentration in breast milk / pregnant woman {{custom_keyword}} /
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温州市医药卫生科学研究项目（编号：2015B53）
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